a second generation platelet concentrate


LL-PRF™ is a three-dimensional autogenous combination of Platelet Rich Fibrin derived from the patient’s blood. A simplified chairside procedure results in the production of a thin, compressed layer of platelet rich fibrin that is strong, pliable and suitable for suturing. This natural fibrin network is rich in platelets, growth factors and cytokines that are derived from the blood platelets and leukocytes [1]. The presence of these proteins have been reported to produce rapid healing, especially during the critical first seven days after placement [2]. This network promotes more efficient cell migration and proliferation without chemical or bovine thrombin additives [3].

Excellent handling properties and no biomedical modifications

Clinically, Leukocyte-Platelet Rich Fibrin displays excellent working properties. This biomaterial is resilient and easy to manipulate. It can be cut to size, and is supple enough to adapt to many anatomical areas. It is adhesive in nature and very receptive to suturing. In addition, there is ample working time since L-PRF™ is stable at room temperature for several hours [4].

The IntraSpin™ System is intended to be used for the safe and rapid preparation of autologous Platelet Rich Fibrin (PRF) from a small sample of blood taken at the patient’s point of care. L-PRF™ can be mixed with all bone grafts prior to application to a bony defect for improving handling characteristics. It requires only one centrifugation without pipetting, mixing, heating or additives. Every component of the IntraSpin™ System has been specifically selected and engineered to act in concert as a graft delivery.


In general, L-PRF™ can be applied in all dental indications to support wound healing. In addition, L-PRF™ may be used in combination with bone grafts to improve their handling properties and to promote their integration [5,6].

  • Ridge augmentations to cover the augmented area
  • Sinus floor elevation
  • Extraction sockets
  • Recession coverage
  • Wound management after surgical interventions
  • Coating of implants


  • Original L-PRF™ protocol as described in the literature [1]
  • Simple and ergonomic, i.e. no pipetting, no second spin, no heating steps
  • 100% natural, i.e. no anticoagulants, no chemical additives
  • Slow and sustained release of growth factors for > 7 days [2]
  • Reduced pain und patient discomfort [7,8]
  • Improved wound healing [9]

More Events on botiss-academy


Socket preservation using L-PRF
Immediate implant placement using L-PRF

Prof. David M. Dohan Ehrenfest
Research Center for Biomineralization Disorders, Chonnam National University School of Dentistry, Gwangju, South Korea
Department of Oral and Maxillofacial Surgery, University of Michigan Health System, Ann Arbor, USA

Dr. Marco Del Corso, Private practice, Turin, Italy

Protection of the Schneiderian membrane using L-PRF during sinus floor elevation with simultaneous implant placement

Dr. Anke Isser
Private practice
Frankfurt am Main



Simple and reproducible chairside procedure

The IntraSpin™ System establishes a three-step protocol for drawing and centrifuging the patient’s blood, removing the fibrin clot and processing it in the Xpression™ Fabrication Kit. A thin, compressed layer of Platelet Rich Fibrin or plugs for extraction sites can then be formed, using either the internal plate or the piston assembly.

Trouble shooting

Blood should be drawn as quickly as possible (ideally within less than one minute) followed by immediate centrifugation to yield a big, well-defined fibrin clot. A delay may affect the separation of the blood components and the formation of the clot. Anticoagulants interfere with the coagulation process which may result in delay of the clotting. If patients are on any type of anticoagulant therapy, the centrifugation time should be prolonged to 18 min. In addition, imbalance may cause vibrations that will transmit to the tubes and will disturb coagulation and separation of the blood components. Centrifuge tubes always in pairs and place them in opposite positions to balance the rotor.

Storage of the matrices

After processing, the L-PRF™ fibrin matrix can be stored in the Xpression™ Fabrication Box for up to 3 hours without compromising cell vitality and fibrin architecture. In general, it is recommended to prepare the L-PRF™ fibrin matrix before surgery.

Eight matrices at the same time

The IntraSpin™ centrifuge can harbor eight blood collection tubes at the same time. As one tube practically yields one fibrin clot and as the Xpression™ Fabrication Box can harbor eight fibrin clots, virtually eight L-PRF™ fibrin matrices are possible to produce during a single preparation step.

Barrier membrane

L-PRF™ is used to stimulate tissue regeneration and wound healing. It can be considered an optimized blood clot that will be rapidly remodeled at wound and augmentation sites. Barrier membranes instead are intended to provide space and to separate fast from slow growing cells in the sense of Guided Tissue and Guided Bone Regeneration (GTR, GBR). Hence, an additional use of a barrier membrane in GTR and GBR procedures is highly recommended.

CE clearance

The IntraSpin™ L-PRF system, including the provided centrifuge and tubes, is designed for in vivo use and is registered as medical device with CE clearance according to the European directive 93/42/EEC.

Product Specifications

ISS220IntraSpin™ System
IntraSpin™ Centrifuge1 ×
Vacuum Blood Collection Tubes Red100 x
Butterfly Blood Collection Sets24 x
Tourniquet1 x
Xpression™ Fabrication Box1 x
Surgical Tissue Forceps1 x
Surgical Curved Scissors1 x
Dual Biomaterial Carrier Spatula1 x
Rectangular Stainless Steel Bowl1 x
Round Stainless Steel Bowl1 x
Dual Biomaterial Packer1 x
Tube Rack1 x
L-PRF Protocol1 x
 CTRXpression™ Fabrication Box (w/o instruments)
1 x
 LPRF 455385Vacuum Blood Collection Tubes Red
100 x
 LPRF 455006Vacuum Blood Collection Tubes White
100 x
 PRF450Butterfly Blood Collection Sets
24 x


  • Dohan Ehrenfest DM, Kang BS, Del Corso M, Nally M, Quirynen M, Wang HL, Pinto NR. The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors and fibrin architecture of a Leukocyte- and Platelet-Rich Fibrin (L-PRF) clot and membrane. Part 1: evaluation of the vibration shocks of 4 models of table centrifuges for L-PRF. POSEIDO. 2014;2(2):129-39. LINK
  • Pinto NR, Pereda A, Jiménez P, Del Corso M, Kang BS, Wang HL, Quirynen M, Dohan Ehrenfest DM. The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors and fibrin architecture of a Leukocyte- and Platelet-Rich Fibrin (L-PRF) clot and membrane. Part 2: macroscopic, photonic
    microscopy and Scanning Electron Microscopy analysis of 4 kinds of L-PRF clots and membranes. POSEIDO. 2014;2(2):141-54. LINK
  • Dohan Ehrenfest DM, Del Corso M, Kang BS, Lanata N, Quirynen M, Wang HL, Pinto NR. The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors and fibrin architecture of a Leukocyte- and Platelet-Rich Fibrin (L-PRF) clot and membrane. Part 3: comparison of the growth factors content and slow release between the original L-PRF and the modified A-PRF (Advanced Platelet-Rich Fibrin) membranes. POSEIDO. 2014;2(2):155-66. LINK

Let us know YOUR COUNTRY and we will provide you with YOUR right local CONTACT PERSON!

Email to product-management@botiss.com!


[1] Dohan Ehrenfest DM et al. J Periodontol. 2010
[2] Dohan Ehrenfest DM et al.  Growth Factors. 2009
[3] Dohan DM et al. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006
[4] Toffler et al. JIACD 2009
[5] Fernando & David Philipp J Otolaryngol Head Neck Surg 2013
[6] Nacopoulos et al. J Craniofac Surg. 2014
[7] Marenzi et al. BioMed Research International 2015
[8] Uyanık et al. Head Face Med. 2015
[9] Munoz et al. J Clin Exp Dent. 2016